Mrs. Diana Solovyov - M.Sc. Candidate
23/03/2025
אודיטוריום ע"ש דויד וואנג, בניין מידן, קומה 3
13:30
Biocompatible polysaccharide-based injectable hydrogels offer a promising approach for enhancing stem cell differentiation and promoting soft tissue regeneration. This project designs and characterizes injectable hydrogels made of hydrolyzed levan, a biocompatible polymer composed of fructose moieties, loaded with cannabidiol (CBD), a cannabinoid with anti-inflammatory and tissue-healing properties, for localized gingival wound treatment. First, the hydrogels were synthesized and chemically crosslinked for structural stability. Their physical properties, including water absorption and retention, were analyzed to ensure a hydrated environment conducive to tissue regeneration. Due to CBD’s poor water solubility and sensitivity to environmental conditions, it was encapsulated within polymeric micelles before being incorporated into the hydrogels. The release profile showed an initial burst followed by gradual, sustained release. The interaction of the hydrogels with patient-derived gingival mesenchymal stem cells (GMSCs) was investigated. Initial tests confirmed very good cell compatibility within three days, prompting further evaluation of prolonged exposure to simulate tissue regeneration. Cell viability remained stable over 28 days, allowing a deeper analysis of hydrogel effects on GMSC differentiation. Further studies are ongoing to confirm molecular-level effects. These findings highlight the potential of polysaccharide-based injectable hydrogels as a minimally invasive alternative to grafting, offering controlled bioactive release and enhanced regenerative outcomes.
Supervisor: Prof. Alejandro Sosnik