Mrs. Hen Moshe Halamish PhD candidate
David Wang Auditorium, 3rd floor Dalia Meidan Bldg.
Drug nano-encapsulation has been investigated to prevent premature degradation, enhance bioavailability and efficacy and the ability to target it to specific body sites. In our attempt to target cancer cells that overexpress sialic acid, we synthesized and characterized a novel family of amphiphilic PVA-based nanoparticles (NPs) that were physically stabilized by boric acid crosslinking which also confers a borated surface. Non-crosslinked and crosslinked NPs (diameter of 70-200 nm, as measured by dynamic light scattering) usually show narrow and monomodal size distribution, and good cell compatibility. In addition, they show substantially greater (50-fold) encapsulation capacity for the tyrosine kinase inhibitor dasatinib with respect to other polymeric amphiphilic nanoparticles and can be spray-dried and redispersed without losing their properties. Interestingly, boric acid-crosslinked NPs show better permeability and uptake by a 3D spheroid model of rhabdomyosarcoma (a pediatric sarcoma) that overexpress sialic acid and result in an increase of the anti-cancer activity in vitro. Finally, the tumor targeting of non-crosslinked and crosslinked nanocarriers was compared in a subcutaneous xenograft murine model of rhabdomyosarcoma. Crosslinked NPs mainly accumulated in the tumor, as opposed to the non-crosslinked counterparts that could be detected in the liver, a major clearance organ. Overall, results highlight the promise of these nanocarriers for the targeting of tissues overexpressing sialic acid.
BSc- Materials Science and Engineering, Technion
BSc- Chemistry, Technion
PhD (direct track) – Materials Science and Engineering, Technion