Dr. Urszula (Ula) Stachewicz
David Wang Auditorium, 3rd floor Dalia Meidan Bldg.
Electrospun polymer fibers are one most studied material for tissue engineering as it is characterized by high surface area to the mass ratio. The porosity of electrospun scaffolds reaches often more than 90 %, which gives an enormous advantage in tissue engineering. Apart from porosity, the key parameters in biomedical applications are surface properties, which we control via a single-step electrospinning process by alternating voltage polarities. Voltage polarity defines the charge accumulated on the surface of the liquid jet and the surface of the fibers. Positive polarity attracts negatively charged groups to fibers’ surface, whereas negative polarity moves the negatively charged functional groups away from the surface. This way we can control the surface chemistry, wettability and additionally surface potential of electrospun fibers. We proved the significant effect of fibers surface potential and geometry on cell integration with the scaffolds and further cell development in the regeneration processes based on the osteoblast, fibroblast and keratinocytes culture studies.
Additionally, the controlled geometries of electrospun fibers and membranes show a great application possibility as skin patches. The experimental studies in vitro and in vivo have been confirmed with the numerical simulations of oil transport through the porous patches. We show that oil spreading and transport can be controlled and results in increased skin hydration. The electrospun patches can be easily applied overnight on skin with atopic dermatitis.
Supervisor: Asst. Prof. Yonatan Calahorra